Abstract- There is increasing reports of emergence of multiple drug resistant (MDR) Acinetobacter spp. in the world. A. baumannii isolates from JJM Medical College hospital were studied in order to find the profile of antibiotic resistance. Among them, 40.6% (59/145) of A. baumannii isolates were identified as MDR. Overall susceptibility rates to ofloxacin, gentamicin, carbenicillin, cephotaxime, ceftazidime and ceftriaxone were 45.45%, 38.96%, 42.86%, 41.56%, 50.65% and 29.87%, respectively. The susceptibility rates to amikacin, ciprofloxacin, imipenem, and meropenem were 57.14%, 58.44%, 67.53% and 71.42% respectively. Our findings also highlight the importance of clinicians’ access to updated susceptibility data regarding A.baumannii in developing countries such as India.