Chemopreventive potential of tinospora cordifolia root extract against aflatoxin b1-induced toxicity in swiss albino mice

Abstract

The present investigation aims at assessing the chemopreventive potential of ethanolic extract of traditional medicinal plant, Tinospora cordifolia against aflatoxin-B1 (AFB1) induced hepatic and renal damage in mice. The results of present study revealed that administration of AFB1 induced oxidative stress in liver and kidney of treated mice through elevating the level of malondialdehyde (MDA) and depleting the levels enzymatic (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic (reduced glutathione, vitamin C and protein) antioxidants in hepatic and renal tissues. AFB1 supplementation also showed significant increase in serum marker enzymes and influenced the hematological variables. Administration of low, medium and high dose of ethanolic extract of Tinospora cordifolia effectively ameliorated the deviation induced in liver and kidney tissues of mice in response to aflatoxin administration. This effect was evident through reducing MDA level and releasing the inhibitory effect of aflatoxin on levels of antioxidant in liver and kidney tissues. However, administration of plant extract along with aflatoxin restores the damages caused by aflatoxin in hematological and serological variables. Pathological examination of liver and kidney tissues also supported the biochemical findings. From the current investigation it can be concluded that supplementation of plant extract was beneficial in modulating the alteration induced in liver, kidney, serum and blood variables of mice under the effect of AFB1.