Antioxidant activity of leaf different extracts in punica granatum

Abstract

Background: Pomegranate (Punica granatum L.) is an edible fruit native to Persia that is grown and consumed around the world, including the Iran; it has been revered through the ages for its medicinal properties. In the present project, we reported the research on different extracts of different polarity from leaves for their antioxidant activity. Methods: Pomegranate leaves were extracted with petroleum ether. The dried petroleum extract was suspended in ethanol %80 and successively partitioned with n-butanol. Three different bioassays were used, namely scavenging of the β-carotene-linoleic acid bleaching method, nitric oxide scavenging and hydroxyl oxide cavenging. Results: Total phenolic content was quantified as well. All statistical analyses were carried out with P values <0.001 were considered significant. The results of this study suggested that the antioxidant activities of Pomegranate leaves have direct relationship with phenolic compounds. Thus, extract concentration have significant relationship in different tests. Conclusions: Antioxidant activity of plant extracts is not limited to phenolics; the presence of different antioxidant components in the extracts that function as hydrogen donors, may contribute to the concentration of the total phenols determined. Therefore, there is no relationship between the concentration of polyphenol and the antioxidant activity when comparing plant extracts.